Histopathological Test of Placental Tissue to Measure the Effectiveness of Mahkota Dewa Fruit (Phaleria Macrocarpa) in Overcoming Placental Inflammation in Case of Preeclampsia
DOI:
https://doi.org/10.37287/ijghr.v8i3.1844Keywords:
histopathology, mahkota dewa, phaleria macrocarpa, inflammation placenta, preeclampsiaAbstract
Preeclampsia is disturbance pregnancy - related hypertension close with inflammation placenta, stress oxidative, and damage structure network placenta that has an impact bad to health mother and fetus. Available therapies moment This Still focus on control symptoms and not yet in a way specific target mechanism base in the form of inflammation placenta. Fruit The Crown of God (Phaleria macrocarpa) is plant drug original Indonesian containing compound bioactive potential anti-inflammatory, but proof scientific about its effectiveness to inflammation placenta in preeclampsia Still limited. Research objectives This For test effectiveness extract fruit Crown of the Gods in lower inflammation placenta based on description histopathology network placenta in preeclampsia model. Research methods This use design true experimental post-test only control group in mice white female induced pregnancy (Rattus norvegicus) preeclampsia using monosodium glutamate. Subject study shared become six group, namely normal control, control preeclampsia, control nifedipine comparator, as well as three group treatment extract ethanol fruit Crown of the Gods with doses of 180, 360, and 720 mg/ kgBW. Tissue placenta taken after delivery and examination in a way histopathology use coloring hematoxylin -eosin with infiltration parameters cell inflammation, edema, necrosis, degeneration trophoblast, and cell structure. Data were analyzed using one ANOVA test direction and Tukey's further test. The results of the study show that induction preeclampsia cause improvement inflammation real placenta, whereas giving extract fruit Crown of the Gods in a way significant lower degrees inflammation placenta. Dose 360 mg/ kgBW show effectiveness best with description histopathology that approaches normal conditions. It was concluded that fruit The Crown of God is proven effective lower degrees damage histopathological placenta in a way significant in the preeclampsia model.
References
Abdur Rashid Mia, M., Uddin Ahmed, Q., Ferdosh, S., Bashar Mohammed Helaluddin, A., Najmul Hejaz Azmi, S., Ammar Al-Otaibi, F., Parveen, H., Mukhtar, S., Ahmed, M. Z., & Zaidul Islam Sarker, M. (2023). Anti-obesity and antihyperlipidemic effects of Phaleria macrocarpa fruit liquid CO2 extract: In vitro, in silico and in vivo approaches. Journal of King Saud University - Science, 35(8). https://doi.org/10.1016/j.jksus.2023.102865
Ahmad, R., Khairul Nizam Mazlan, M., Firdaus Abdul Aziz, A., Mohd Gazzali, A., Amir Rawa, M. S., & Wahab, H. A. (2023). Phaleria macrocarpa (Scheff.) Boerl.: An updated review of pharmacological effects, toxicity studies, and separation techniques. In Saudi Pharmaceutical Journal (Vol. 31, Number 6, pp. 874–888). Elsevier B.V. https://doi.org/10.1016/j.jsps.2023.04.006
Bacmeister, L., Buellesbach, A., Glintborg, D., Jorgensen, J. S., Møller Luef, B., Birukov, A., Heidenreich, A., Lindner, D., Keller, T., Kraeker, K., Zeller, T., Dechend, R., Skovsager Andersen, M., & Westermann, D. (2025). Third-Trimester NT-proBNP for Pre-eclampsia Risk Prediction: A Comparison With sFlt-1/PlGF in a Population-Based Cohort. JACC: Advances, 4(4). https://doi.org/10.1016/j.jacadv.2025.101671
Bucher, V., Mitchell, A. R., Gudmundsson, P., Atkinson, J., Wallin, N., Asp, J., Sennström, M., Hildén, K., Edvinsson, C., Ek, J., Hastie, R., Cluver, C., & Bergman, L. (2024). Prediction of adverse maternal and perinatal outcomes associated with pre-eclampsia and hypertensive disorders of pregnancy: a systematic review and meta-analysis. www.thelancet.com
Burton, G. J., Jauniaux, E., & Moffett, A. (2025). Formation of the placental membranes and pathophysiological origin of associated great obstetrical syndromes. In American Journal of Obstetrics and Gynecology. Elsevier Inc. https://doi.org/10.1016/j.ajog.2025.07.039
Christina, Y. I., Rifa’i, M., Widodo, N., & Djati, M. S. (2022). The combination of Elephantopus scaber and Phaleria macrocarpa leaves extract promotes anticancer activity via downregulation of ER-α, Nrf2 and PI3K/AKT/mTOR pathway. Journal of Ayurveda and Integrative Medicine, 13(4). https://doi.org/10.1016/j.jaim.2022.100674
Danielli, M., Thomas, R. C., Gillies, C. L., Hu, J., Khunti, K., & Tan, B. K. (2022). Blood biomarkers to predict the onset of pre-eclampsia: A systematic review and meta-analysis. Heliyon, 8(11). https://doi.org/10.1016/j.heliyon.2022.e11226
Fernenda, L., Putriani, K., & Apriliani, O. (2024). Toxicity Test of 96% Ethanol Extract of Mahkota Dewa Leaves (Phaleria Macrocarpa) using the Brine Shrimp Lethality Test Method Uji Toksisitas Ekstrak Etanol 96% Daun Mahkota Dewa (Phaleria Macrocarpa) dengan Metode Brine Shrimpe Lethality Test (BSLT). Jurnal Ilmu Kesehatan Abdurrab), 2(1), 10–17.
Gatford, K. L., Andraweera, P. H., Roberts, C. T., & Care, A. S. (2020). Animal Models of Preeclampsia: Causes, Consequences, and Interventions. In Hypertension (Vol. 75, Number 6, pp. 1363–1381). Lippincott Williams and Wilkins. https://doi.org/10.1161/HYPERTENSIONAHA.119.14598
Hurrell, A., Webster, L., Sparkes, J., Battersby, C., Brockbank, A., Clark, K., Duhig, K. E., Gill, C., Green, M., Hunter, R. M., Seed, P. T., Vowles, Z., Myers, J., Shennan, A. H., Chappell, L. C., Ahuja, A., Alexander, H., Arya, R., Bahl, R., … Yulia, A. (2024). Repeat placental growth factor-based testing in women with suspected preterm pre-eclampsia (PARROT-2): a multicentre, parallel-group, superiority, randomised controlled trial. The Lancet, 403(10427), 619–631. https://doi.org/10.1016/S0140-6736(23)02357-7
Mia, M. A. R., Ahmed, Q. U., Helaluddin, A. B. M., Ferdosh, S., Siddique, M. M., Azmi, S. N. H., Ahmed, J., & Sarker, M. Z. I. (2022). Acute and subacute toxicity assessment of liquid CO2 extract of Phaleria macrocarpa fruits flesh in mice model. Journal of King Saud University - Science, 34(4). https://doi.org/10.1016/j.jksus.2022.101912
Nakimuli, A., Akello, J., Sekikubo, M., Nakubulwa, S., Adroma, M., Nabuufu, R., Obuya, E., Bagala, J. P., Twinamatsiko, A., Nakatudde, H., Pirio, P., Latigi, G., Arwinyo, B., Mugabe, K., Chebet, I., Mugahi, R., Aitchison, I., Patient, C., Moffett, A., & Aiken, C. E. (2025). Variations in emergency care for severe pre-eclampsia in Uganda: a national evaluation study. AJOG Global Reports, 5(1). https://doi.org/10.1016/j.xagr.2024.100424
Obeagu, E. I., & Obeagu, G. U. (2024). Hypoxia-Induced Inflammation: Implications for Maternal Health. In Elite Journal of Scientific Research and Review (Vol. 2, Number 6). https://epjournals.com/journals/EJSRR
Putri, D., Wirasutisna, K. R., mariani, R., Wibowo, D. P., Studi Farmasi, P., & Tinggi Farmasi Indonesia, S. (n.d.). Etnofarmakognosi, Kandungan Kimia serta Aktivitas Farmakologi Tanaman Mahkota Dewa (Phaleria macrocarpa (Scheff) Boerl) : Literature Review Ethnopharmacognosy, Chemical Content and Pharmacological Activity of Mahkota Dewa Plant (Phaleria macrocarpa (Scheff) Boerl): Literature Review. 6. https://doi.org/10.56338/jks.v6i12.4458
Raghu, R., Kurlak, L. O., Lee, E. D., & Mistry, H. D. (2023). The differential placental expression of ERp44 and pre-eclampsia; association with placental zinc, the ERAP1 and the renin-angiotensin-system. Placenta, 134, 9–14. https://doi.org/10.1016/j.placenta.2023.02.006
Simanjuntak, L. J., & Rumahorbo, C. G. P. (2024). Nano-based herbal Mahkota Dewa (Phaleria macrocarpa) for pre-eclampsia: A histological study on placental and blood changes. Journal of King Saud University - Science, 36(11). https://doi.org/10.1016/j.jksus.2024.103534
Sukma, U. F. (2020). Aktivitas Antihipertensi Fraksi N-Heksan Ekstrak Etanol Labu Siam (Sechium edule (Jack) Sw) Pada Tikus Hipertensi Yang Diinduksi Monosodium Glutamat (MSG) SKRIPSI.
Tong, H., Wang, T., Chen, R., Jin, P., Jiang, Y., Zhang, X., & Qi, H. (2024). High level 27-HC impairs trophoblast cell invasion and migration via LXR in pre-eclampsia. Experimental Cell Research, 437(1). https://doi.org/10.1016/j.yexcr.2024.113979
Tong, W., Allison, B. J., Brain, K. L., Patey, O. V., Niu, Y., Botting, K. J., Ford, S. G., Garrud, T. A., Wooding, P. F. B., Shaw, C. J., Lyu, Q., Zhang, L., Ma, J., Cindrova-Davies, T., Yung, H. W., Burton, G. J., & Giussani, D. A. (2022). Chronic Hypoxia in Ovine Pregnancy Recapitulates Physiological and Molecular Markers of Preeclampsia in the Mother, Placenta, and Offspring. Hypertension, 79(7), 1525–1535. https://doi.org/10.1161/HYPERTENSIONAHA.122.19175
Vlachodimitropoulou, E., Balasubramanian, T., Shehata, N., Ward, R., Kuo, K. H. M., Kingdom, J., & Malinowski, A. K. (2024). The Utility of Maternal Placental Growth Factor (PlGF) for Prediction of Pre-Eclampsia in Pregnancies Complicated By Sickle Cell Disease. Blood, 144(Supplement 1), 1118–1118. https://doi.org/10.1182/blood-2024-206330
Vornic, I., Buciu, V., Furau, C. G., Gaje, P. N., Ceausu, R. A., Dumitru, C. S., Barb, A. C., Novacescu, D., Cumpanas, A. A., Latcu, S. C., Cut, T. G., & Zara, F. (2024). Oxidative Stress and Placental Pathogenesis: A Contemporary Overview of Potential Biomarkers and Emerging Therapeutics. In International Journal of Molecular Sciences (Vol. 25, Number 22). Multidisciplinary Digital Publishing Institute (MDPI). https://doi.org/10.3390/ijms252212195
Zhang, J., Lu, Y., Li, L., Li, X., Ying, J., Li, S., Wu, L., & Li, L. (2024). Pre-eclampsia intronic polyadenylation enriched in VEGFA-VEGFR2 signaling pathway. Heliyon, 10(21). https://doi.org/10.1016/j.heliyon.2024.e39495
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