Physicochemical Characteristics and Antioxidant Activity of Sacha Inchi (Plukenetia volubilis L.) Seed Oil: Effect of Microwave Roasting Pre-Treatment

Authors

  • Ulan Mulyani Saputri Universitas Halu Oleo
  • Irnawati Irnawati Universitas Halu Oleo
  • Asriullah Jabbar Universitas Halu Oleo
  • Sahidin Sahidin Universitas Halu Oleo
  • Nasrudin Nasrudin Universitas Halu Oleo
  • Ari Sartinah Universitas Halu Oleo
  • Awal P. Kusumadewi Medicinal Plant and Traditional Medicine Research and Development Center

DOI:

https://doi.org/10.37287/ijghr.v8i5.2108

Keywords:

IC50, physicochemical characteristics, roasting pre-treatment, sacha inchi (Plukenetia volubilis L.)

Abstract

Sacha inchi seed oil (Plukenetia volubilis L.) has considerable potential as a functional oil source due to its high content of unsaturated fatty acids and bioactive compounds. The processing method, especially the roasting pre-treatment, can affect the quality and stability of the resulting oil, both in terms of physicochemical characteristics and antioxidant activity. This study aims to evaluate the effect of microwave roasting pre-treatment on the physicochemical characteristics and antioxidant activity of Sacha inchi seed oil extracted using the Soxhlet method.  Roasting was carried out at Med-Low 30P, Medium 50P, and Med-High 70P power levels. The oil was extracted using the Soxhlet method. Physicochemical properties, including acid value, peroxide value, iodine value, and saponification value, were analyzed using AOCS standard methods, while antioxidant activity was evaluated spectrophotometrically using DPPH, ABTS, and β-carotene bleaching assays methods. The results showed that increasing roasting power increased oil yield, with the highest yield (62%) obtained at Med-High 70P treatment. Variation in roasting power did not have a significant effect on acid value (1.301–1.418 mg KOH/g oil) and iodine value (101.940–103.883 g I₂/100 g oil), but had a significant effect on peroxide value (5.32–5.82 meq O₂/kg) and saponification value (171.591–180.422 mg KOH/g oil). The highest antioxidant activity was obtained at Medium 50P treatment for the β-carotene bleaching assay (501.58 mg/L) and DPPH (1214.92 mg/L) methods, and at Med-Low 30P treatment for the ABTS method (673.70 mg/L). Microwave roasting pre-treatment affects the increase in yield as well as several physicochemical parameters and antioxidant activity of sacha inchi seed oil.

References

Alkaltham, M. S., Özcan, M. M., Uslu, N., Salamatullah, A. M., & Hayat, K. (2020). Effect of microwave and oven roasting methods on total phenol , antioxidant activity , phenolic compounds , and fatty acid compositions of coffee beans. Food Processing and Preservation, January, 1–9. https://doi.org/10.1111/jfpp.14874

Amakhmakh, M., Hajib, A., Belmaghraoui, W., Harhar, H., Asri, E., Al, W., Wen, K., & Bouyahya, A. (2024). Food Chemistry : X Assessment of the impact of microwave roasting on nutrient content , lipid profile , and oxidative stability of pomegranate seed oil. Food Chemistry: X, 24(October), 101875. https://doi.org/10.1016/j.fochx.2024.101875

AOAC. (1990). OFFICIAL METHODS OF ANALYSIS (K. Helrich (ed.); 15th ed.). The Association of Analytical Chemists.

Bakhshabadi, H., Ganje, M., Gharekhani, M., Mohammadi-moghaddam, T., Aulestia, C., & Morshedi, A. (2025). A Review of New Methods for Extracting Oil from Plants to Enhance the Efficiency and Physicochemical Properties of the Extracted Oils. Processes, 13, 1124. https://doi.org/https://doi.org/10.3390/pr13041124

Bakhshabadi, H., Mirzaei, H., Ghodsvali, A., Mehdi, S., Mohammad, A., & Farzaneh, V. (2017). The effect of microwave pretreatment on some physico-chemical properties and bioactivity of Black cumin seeds ’ oil. Industrial Crops & Products, 97, 1–9. https://doi.org/10.1016/j.indcrop.2016.12.005

Borges, L. B., Marco, A. S., Claudia, C. G., Suely, V. S., Paulo, H. V. R., & Marisa, A. B. R. (2018). Sacha inchi seeds from sub-tropical cultivation : effects of roasting on antinutrients , antioxidant capacity and oxidative stability. https://doi.org/10.1007/s13197-018-3345-1

Chan, Y., Chiu, C., Li, P., & Lu, W. (2024). Evaluation of different roasting condition on yield , physico-chemical characteristics , and antioxidant activity of cold-pressed sacha inchi ( Plukenetia volubilis ) oil. LWT - Food Science and Technology, 203(June). https://doi.org/https://doi.org/10.1016/j.lwt.2024.116343

Chinh, H., Phu, D., Thanh, N., Nguyen, T., Phuong, N., Su, C., Wang, F., & Juan, H. (2020). Aqueous enzymatic extraction of polyunsaturated fatty acid – rich sacha inchi ( Plukenetia volubilis L .) seed oil : An eco-friendly approach. LWT, 133(July), 109992. https://doi.org/10.1016/j.lwt.2020.109992

Chirinos, R., Pedreschi, R., Domínguez, G., & Campos, D. (2015). Comparison of the physico-chemical and phytochemical characteristics of the oil of two Plukenetia species. FOOD CHEMISTRY, 173, 1203–1206. https://doi.org/10.1016/j.foodchem.2014.10.120

Christopher, J., Tan, C. P., Wasoh, H., & Lai, O. M. (2023). Comparative Study of Thermal Pre-treatment on the Extraction, Antioxidant, Fatty Acid Profile, and Physicochemical Properties of Inca Inchi Seed Oil. Pertanika Journal of Tropical Agricultural Science, 46(3), 909–930. https://doi.org/10.47836/pjtas.46.3.11

Codex, A. (2024). STANDARD FOR NAMED VEGETABLE OILS CXS 210-1999. Codex Alimentarius Standard.

Fong, Y. K., Muhamad, N., Salleh, L. M., & Abang Zaidel, D. N. (2023). Microwave Drying of Sacha Inchi (Plukenetia Volubilis L.) Seed for Improving Oil Yield Extract. Journal of Bioprocessing and Biomass Technology, 2(2), 7–11. https://doi.org/10.11113/bioprocessing.v2n2.29

Gaber, M. A. F. M., Mansour, M. P., Trujillo, F. J., & Juliano, P. (2020). Microwave pre-treatment of canola seeds and flaked seeds for increased hot expeller oil yield. Journal of Food Science and Technology. https://doi.org/10.1007/s13197-020-04545-9

Hamdi, A., Majouli, K., Flamini, G., Marzouk, B., Marzouk, Z., & Heyden, Y. V. (2017). South African Journal of Botany Antioxidant and anticandidal activities of the Tunisian Haplophyllum tuberculatum ( Forssk .) A . Juss . essential oils. South African Journal of Botany, 112, 210–214. https://doi.org/10.1016/j.sajb.2017.05.026

Karrar, E., Ahmed, I. A. M., Manzoor, M. F., AL-Farga, A., Wei, W., Albakry, Z., Sarpong, F., & Wang, X. (2021). Effect of roasting pretreatment on fatty acids, oxidative stability, tocopherols, and antioxidant activity of gurum seeds oil. Biocatalysis and Agricultural Biotechnology, 34(February), 1–7. https://doi.org/10.1016/j.bcab.2021.102022

Kaseke, T., Opara, U. L., & Fawole, O. A. (2020). Effect of Microwave Pretreatment of Seeds on the Quality and Antioxidant Capacity of Pomegranate. Foods, 9. https://doi.org/doi:10.3390/foods9091287

Kim, D., & Joo, N. (2019). Nutritional composition of Sacha inchi ( Plukenetia Volubilis L .) as affected by different cooking methods Nutritional composition of Sacha inchi ( Plukenetia Volubilis L .) as. International Journal of Food Properties, 22(1), 1235–1241. https://doi.org/10.1080/10942912.2019.1640247

Kittibunchakul, S., Kemsawasd, V., Hudthagosol, C., Sanporkha, P., Sapwarobol, S., & Suttisansanee, U. (2023). The Effects of Different Roasting Methods on the Phenolic Contents, Antioxidant Potential, and In Vitro Inhibitory Activities of Sacha Inchi Seeds. Foods, 12(22). https://doi.org/10.3390/foods12224178

Kong, S., Keang, T., Bunthan, M., Say, M., Nat, Y., Tan, C. P., & Tan, R. (2023). Hydraulic Cold-Pressed Extraction of Sacha Inchi Seeds : Oil Yield and Its Physicochemical Properties.

Konuskan, D. B., Arslan, M., & Oksuz, A. (2019). Physicochemical properties of cold pressed sunflower, peanut, rapeseed, mustard and olive oils grown in the Eastern Mediterranean region. Saudi Journal of Biological Sciences, 26(2), 340–344. https://doi.org/10.1016/j.sjbs.2018.04.005

Kulik, K. (2025). The Effect of Roasting on the Health-Promoting Components of Nuts Determined on the Basis of Fatty Acids , Polyphenol Compounds , and Antioxidant Capacity. Molecules, 30, 5–10. https://doi.org/https://doi.org/10.3390/molecules30234594

Kyaw, T., Nwe, T. L., Myint, O., Khaing, M. T., San, P. P., Kyaing, K. H., Mon, T. T., & Htun, E. T. (2019). Studies on Nutritional Compositions of Sacha Inchi Seed and Physicochemical Characteristics of Sacha Inchi Oil. 7(8), 111–119.

Maya, I., Winardi, D. O., Amalia, E., Mita, S. R., Kusumawulan, C. K., Putriana, N. A., & Sriwidodo, S. (2023). Physicochemical Characteristics, Fatty Acid Profile, and In Vitro Antioxidant Activity Evaluation of Sacha Inchi Seed Oil from Indonesia. Cosmetics, 10(6). https://doi.org/10.3390/cosmetics10060171

Mohammed, K., Koko, M., Obadi, M., Letsididi, K. S., Cao, P., & Liu, Y. (2017). Effects of Microwave Roasting Process and Time on Chemical Composition and Oxidative Stability of Sunflower Oil. Journal of Food and Nutrition Research, 5(9), 659–667. https://doi.org/10.12691/jfnr-5-9-5

Muangrat, R., Veeraphong, P., & Chantee, N. (2018). Screw press extraction of Sacha inchi seeds: Oil yield and its chemical composition and antioxidant properties. Journal of Food Processing and Preservation, 42(6), 1–10. https://doi.org/10.1111/jfpp.13635

Oubannin, S., Jadouali, S. M., Atifi, H., Bijla, L., Ibourki, M., Gagour, J., Bouzid, H. A., Aabd, N. A., Bouyahya, A., Harhar, H., Goh, K. W., Ming, L. C., Razi, P., & Gharby, S. (2024). Antioxidant Activity, Physico-Chemical Properties, and Bioactive Compounds of Nigella sativa Seeds and Oil Impacted by Microwave Processing Technique. HELIYON, e37603. https://doi.org/10.1016/j.heliyon.2024.e37603

Özcan, M. M., Al-juhaimi, F. Y., Ahmed, I. A. M., Osman, M. A., & Gassem, M. A. (2018). Effect of different microwave power setting on quality of Chia seed oil obtained in a cold press. Food Chemistry. https://doi.org/10.1016/j.foodchem.2018.11.048

Pisco, E. G., & Lujerio, A. F. O. (2016). Effect of Roasting Method Conventional and Microwave in Colour Beans Inka Peanut ( Plukenetia Volubilis ) for the Production of Cream for Human Consumption. Biological and Chemical Research, 3, 67–74.

Ramadhaningtyas, L., Riyanta, A. B., & Barlian, A. A. (2023). Pengaruh Pra-perlakuan Microwave terhadap Aktivitas Antioksidan Minyak Kemiri dengan Menggunakan Metode DPPH The Effect Of Microwave Pre-treatment On The Antioxidant Activity Of Candlenut Oil Using The DPPH Method. Journal Pharmasci (Journal of Pharmacy and Science), 8(1), 21–27.

Ramos-escudero, F., Gonza´lez-Miret, M. L., Vin˜as-Ospino, A., & Escudero, M. R. (2019). Quality , stability , carotenoids and chromatic parameters of commercial Sacha inchi oil originating from Peruvian cultivars. J Food Sci Technol, 56(November), 4901–4910. https://doi.org/10.1007/s13197-019-03960-x

Soimee, W., Nakyai, W., Charoensit, P., Grandmottet, F., Worasakwutiphong, S., Phimnuan, P., & Viyoch, J. (2020). Evaluation of moisturizing and irritation potential of sacha inchi oil. Journal of Cosmetic Dermatology, 19(4), 915–924. https://doi.org/10.1111/jocd.13099

Tianara, A., Handayani, W., Irsyam, A. S. D., Hariri, M. R., Dewi, A. P., Peniwidiyanti, P., Baidlowi, M. H., Rosleine, D., & Atria, M. (2024). Plukenetia volubilis L.: A New Record of a Cultivated Alien Species in Java. Journal of Tropical Biodiversity and Biotechnology, 9(1). https://doi.org/10.22146/jtbb.84523

Tolouie, H., Mohammadifar, M. A., Ghomi, H., Yaghoubi, A. S., & Hashemi, M. (2018). Research Group for Food Production Engineering , National Food Institute , Technical. Innovative Food Science and Emerging Technologies, 2017, #pagerange#. https://doi.org/10.1016/j.ifset.2018.03.002

Valencia, A., Muñoz, A. M., Ramos-escudero, M., Chavez, K. C., & Ramos-escudero, F. (2024). Carotenoid , Tocopherol , and Volatile Aroma Compounds in Eight Sacha Inchi Seed ( Plukenetia volubilis L .) Oil Accessions. Journal of Oleo Science, 674(5), 665–674. https://doi.org/10.5650/jos.ess23158

Xuan, T. D., Gangqiang, G., Minh, T. N., Quy, T. N., & Khanh, T. D. (2018). An overview of chemical profiles, antioxidant and antimicrobial activities of commercial vegetable edible oils marketed in Japan. Foods, 7(2). https://doi.org/10.3390/foods7020021

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Published

2026-09-26

How to Cite

Saputri, U. M., Irnawati, I., Jabbar, A., Sahidin, S., Nasrudin, N., Sartinah, A., & Kusumadewi, A. P. (2026). Physicochemical Characteristics and Antioxidant Activity of Sacha Inchi (Plukenetia volubilis L.) Seed Oil: Effect of Microwave Roasting Pre-Treatment. Indonesian Journal of Global Health Research, 8(5), 791–800. https://doi.org/10.37287/ijghr.v8i5.2108