Antidiabetic Potential of Combined Extracts of Ageratum Conyzoides and Cyperus Rotundus: in Vivo Study
DOI:
https://doi.org/10.37287/ijghr.v8i2.808Keywords:
Ageratum conyzoides, Cyperus rotundus, diabetes mellitus, antihyperglycemic, synergyAbstract
Long-term use of synthetic antidiabetic drugs often causes side effects, prompting the search for safer herbal alternatives. Ageratum conyzoides and Cyperus rotundus are known for their antihyperglycemic properties, but studies on their combination remain limited. To evaluate the antihyperglycemic effect of combined ethanol extracts of A. conyzoides leaves and C. rotundus rhizomes in streptozotocin (STZ)-induced diabetic rats. Male white rats were divided into nine groups: control, single extract, and combination treatments (100:200 and 150:500 mg/kg BW). Treatments were given orally for 15 days, and blood glucose levels were measured periodically. Data were analyzed using one-way ANOVA and Tukey's post hoc test. The combination of A. conyzoides and C. rotundus (150:500 mg/kg BW) significantly reduced blood glucose levels, comparable to glimepiride (p < 0.05 vs. negative control; p > 0.05 vs. positive control). Single extracts showed moderate effects in a dose-dependent manner. The combination of A. conyzoides and C. rotundus extracts exhibited synergistic antihyperglycemic activity, suggesting potential as a safe phytotherapeutic alternative for diabetes management.
References
Al-awar, M. S. A., & Alqabbani, T. Y. (2021). Anti - diabetic, Antioxidants, Anti - hepatorenaltoxicity Activities of Cyperus rotundus rhizome extract in alloxan - induced diabetic Rats. 8(5), 11530–11545.
Ariastuti, R., Ode, L., Fitrawan, M., Nugroho, A. E., & Pramono, S. (2020). Antidiabetes of Combination of Fractionated-extracts of Andrographis paniculata and Centella asiatica in Neonatal Streptozotocin-induced Diabetic Rats. 31(4), 312–322.
Badan Litbang Kesehatan. (2018). Laporan Riskesdas 2018 Nasional.pdf. In Lembaga Penerbit Balitbangkes (p. hal 156). https://repository.badankebijakan.kemkes.go.id/id/eprint/3514/1/Laporan Riskesdas 2018 Nasional.pdf
C. Pay, T. Watuguly, and S. W. (2022). Potensi Ekstrak Daun Bandotan (Ageratum conyzoides L) Sebagai Obat Diabetes Melitus. Jurnal Biologi Pendidikan Dan Terapan, 9, no, 89–99. https://doi.org/10.30598/biopendixvol9issue1page89-99
Deng, S., Xia, L., Zhu, X., Zhu, J., Cai, M., & Wang, X. (2019). Natural Alpha-Glucosidase Inhibitors Rapid Fishing from Cyperus Rotundus Using Immobilized Enzyme Affinity Screening Combined with UHPLC-QTOF MS. 18(May 2018), 1508–1515. https://doi.org/10.22037/ijpr.2019.1100753
Dinkes Kebumen. (2022). Profil Kesehatan Kabupaten Kebumen Tahun 2022. Kebumen.
Harefa, Sari Kristian, Ujianhati Zega, A. S. B. (2022). Pemanfaatan Daun Bandotan (Ageratum Conyzoides L.) Sebagai Obat Tradisional Di Desa Bawoza’ua Kecamatan Telukdalam Kabupaten Nias Selatan. Jurnal Pendidikan Biologi, 3(1).
IDF. (2021). IDF Diabetes Atlas IDF Diabetes Atlas.
Johnes Kalfari, et al. (2025). Indonesian Journal of Pharmacy and Natural Product. 08.
Nyemb Nyunaï ab, et al. (2009). Research Paper Hypoglycaemic And Antihyperglycaemic Activity Of Ageratum. 6, 123–130.
Pay, C., Wael, S., Program, A., Pendidikan, S., Pattimura, U., Studi, P., Biologi, P., & Pattimura, U. (2022). 1 , 2 , 3 1. 9(November), 89–99.
Pichetkun, V., Ei, H., Khine, E., Srifa, S., Nukulkit, S., Nuengchamnong, N., Hansapaiboon, S., Saenmuangchin, R., Chaotham, C., & Chansriniyom, C. (2024). Diverse effects of a Cyperus rotundus extract on glucose uptake in myotubes and adipocytes and its suppression on adipocyte maturation. Scientific Reports, 1–14. https://doi.org/10.1038/s41598-024-59357-0
Siti Sakinah, et al. (2018). Effects of Ageratum conyzoides Leaf Decoction on Glucose Absorption in Rat ’ s Small Intestine. 5(3), 133–138.
W. Rahmatulloh, M. Ardhiani, I. N. Faridah, H. Dania, L. M. Irham, and D. A. P. (2023). Luaran Terapi dan Kualitas Hidup Pasien DMT2 yang Menggunakan Insulin di Apotek X Kota Yogyakarta. Medical Sains : Jurnal Ilmiah Kefarmasian, 8, no, 395–404. https://doi.org/10.37874/ms.v8i2.742
Woyessa, A. M., Bultum, L. E., & Lee, D. (2024). Designing combinational herbal drugs based on target space analysis. BMC Complementary Medicine and Therapies, 9, 1–15. https://doi.org/10.1186/s12906-024-04455-9.
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2026 Indonesian Journal of Global Health Research
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
