Antidepressant Potential of Etlingera Calophrys in Mice Tail Suspension Test and Forced Swim Test
DOI:
https://doi.org/10.37287/ijghr.v8i4.1967Keywords:
antidepressant, etlingera calophrys, lavender, mice, phytochemicals, tail suspension testAbstract
Depression is a mood disorder characterized by feelings of sadness, loss of interest, and decreased activity, and is associated with neurotransmitter imbalance and oxidative stress. The use of conventional antidepressant drugs still has limitations so that alternatives from natural ingredients such as Etlingera calophrys are needed which are known to contain potential bioactive compounds. This study aims to determine the antidepressant potential of methanol extract of Etlingera calophrys rhizome in mice (Mus musculus) using the Tail Suspension Test (TST) and Forced Swim Test (FST) methods. This study used the Chronic Mild Stress (CMS) model in mice which were divided into several treatment groups, namely normal control, negative control, positive control (amitriptyline 25 mg/kgBW), and extracts with doses of 100, 200, and 300 mg/kgBW. The parameters observed were the duration of immobility in the TST and FST tests. Data were analyzed using One Way ANOVA followed by the Tukey HSD test. Phytochemical screening results showed the presence of alkaloids, flavonoids, tannins, saponins, and terpenoids. In vivo tests showed that all doses of the extract were able to reduce the duration of immobility compared to the negative control, with a dose of 300 mg/kgBW providing the most significant effect and approaching the positive control (p < 0.05). The methanol extract of Etlingera calophrys rhizome has significant antidepressant activity, with a dose of 300 mg/kgBW being the most effective dose. These findings indicate that the methanolic extract of Etlingera calophrys rhizome exhibits significant antidepressant activity, which may be attributed to the synergistic effects of its bioactive compounds through modulation of monoaminergic neurotransmission and antioxidant mechanisms. In conclusion, Etlingera calophrys rhizome has promising potential as a natural antidepressant candidate, warranting further investigation at the molecular and clinical levels.
References
REFERENCES
Buccafusco, J. J. (2009). Methods of behavior analysis in neuroscience. CRC Press.
Castagn, V., Moser, P., Roux, S., & Porsolt, R. D. (2011). Rodent Models of Depression : Forced Swim and Tail Suspension Behavioral Despair Tests in Rats and Mice. April, 1–14. https://doi.org/10.1002/0471142301.ns0810as55
Chan, E. W. C., Lim, Y. Y., Wong, S. K., Lim, K. K., Tan, S. P., Lianto, F. S., & Yong, M. Y. (2009). Effects of different drying methods on the antioxidant properties of leaves and tea of ginger species. Food Chemistry, 113(1), 166–172. https://doi.org/10.1016/j.foodchem.2008.07.090
Cipriani, A., Furukawa, T. A., Salanti, G., Chaimani, A., Atkinson, L. Z., Ogawa, Y., Leucht, S., Ruhe, H. G., Turner, E. H., Higgins, J. P. T., Egger, M., Takeshima, N., Hayasaka, Y., Imai, H., Shinohara, K., Tajika, A., Ioannidis, J. P. A., & Geddes, J. R. (2018). Comparative efficacy and acceptability of 21 antidepressant drugs for the acute treatment of adults with major depressive disorder: a systematic review and network meta-analysis. The Lancet, 391(10128), 1357–1366. https://doi.org/10.1016/S0140-6736(17)32802-7
Cordell, G. A., Quinn-Beattie, M. L., & Farnsworth, N. R. (2001). The potential of alkaloids in drug discovery. Phytotherapy Research, 15(3), 183–205. https://doi.org/10.1002/ptr.890
Cryan, J. F., Markou, A., & Lucki, I. (2002). Assessing antidepressant activity in rodents: Recent developments and future needs. Trends in Pharmacological Sciences, 23(5), 238–245.
Cryan, J. F., Mombereau, C., & Vassout, A. (2005). The tail suspension test as a model for assessing antidepressant activity: Review of pharmacological and genetic studies in mice. Neuroscience and Biobehavioral Reviews, 29(4–5), 571–625.
Gultom, J. M., & Hartika, R. (2019). Phytochemical screening and antioxidant activity of plant extracts. Journal of Pharmaceutical Sciences and Research, 11(4), 1544–1547.
Diah Astari Salam, Ummul Fausiah, Harni Sartika Kamaruddin, Megawati, Nisrina Muslihin, C., & Sabandar, W. (2025). SKRINING FITOKIMIA, AKTIVITAS ANTIOKSIDAN DAN TOKSISITAS AKUT INFUSA BIJI DAN PERIKARP BUAH OLAE (Etlingera calophrys). 9, 47–61.
Harbone, J. . (1998). Phytochemical methods: A guide to modern techniques of plant analysis. Chapman and Hall.
Kumar, S., & Khanum, F. (2012). Neuroprotective potential of phytochemicals. Pharmacognosy Reviews, 6(12), 81–90.
Lacaille-Dubois, M. A., & Wagner, H. (2000). Bioactive saponins from plants: An update. Studies in Natural Products Chemistry, 21, 633–687.
Liu, M. Y., Yin, C. Y., Zhu, L. J., Zhu, X. H., Xu, C., Luo, C. X., … Zhu, D. Y. (2015). Sucrose preference test for measurement of stress-induced anhedonia in mice. Nature Protocols, 10(8), 1181–1191.
Maes, M., Galecki, P., Chang, Y. S., & Berk, M. (2011). A review on oxidative and nitrosative stress in major depression. Progress in Neuro-Psychopharmacology & Biological Psychiatry, 35(3), 676–692.
Mahato, S. B., & Sen, S. (1997). Advances in triterpenoid research. Phytochemistry, 44(7), 1185–1236.
Mahdavi, B. (2014). Chemical composition and biological activity of essential oils from Etlingera species. Industrial Crops and Products, 54, 311–316.
Malhi, G. S., & Mann, J. J. (2018). Depression. The Lancet, 392(10161), 2299–2312. https://doi.org/10.1016/S0140-6736(18)31948-2
Pan, Y., Chen, X. Y., Zhang, Q. Y., & Kong, L. D. (2010). Microglial NLRP3 inflammasome activation mediates depressive-like behavior. Brain, Behavior, and Immunity, 24(1), 1–9.
Porsolt, R. D., Le Pichon, M., & Jalfre, M. (1977). Depression: a new animal model sensitive to antidepressant treatments. 1977 Nature Publishing Group, 266.
Poulsen, A. D. (2007). Etlingera of Borneo. Natural History Publications.
Simaremare, E. S. (2014). Skrining fitokimia ekstrak tanaman obat. Pharmacy Journal, 11(1), 98–107.
Steru, L., Chermat, R., Thierry, B., & Simon, P. (1985). The tail suspension test: A new method for screening antidepressants in mice. psychopharmacology, 85(3), 367–370.
Sun, P., Wang, F., Wang, L., Zhang, Y., Yamamoto, R., Sugai, T., … Zhang, H. (2013). Chronic mild stress models in depression research. Neuroscience Bulletin, 29(6), 803–810.
Salom, A., & Cardoso, A. (2023). Oxidative Stress in Depression : The Link with the Stress Response , Neuroinflammation , Serotonin , Neurogenesis and Synaptic Plasticity.
Syarif, R. A., et al. (2016). Phytochemical screening and antioxidant activity of Etlingera species. Pharmacognosy Journal, 8(3), 258–262.
Ud-Daula, A. F. M. S., & Basher, M. A. (2019). Pharmacological properties of Etlingera species. Journal of Medicinal Plants Research, 13(2), 20–30.
Willner, P. (2016). Neurobiology of Stress Reliability of the chronic mild stress model of depression : A user survey. Neurobiology of Stress. https://doi.org/10.1016/j.ynstr.2016.08.001
Yahya, A., Ibrahim, N., & Aziz, M. (2011). Phytochemical screening of Etlingera species. Journal of Medicinal Plants Research, 5(18), 4469–4473.
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